Morphological and Functional Features of Newborn Piglets Adrenal Cells During Culturing
Abstract
The article contains analysis of morphological and functional features of newborn piglets adrenal cells during culturing. It was shown that the newborn piglets adrenal cell culture consists of monolayer of fibroblast-like cells and spherical cell structures (cytospheres). It was demonstrated that the number of 3-β-HSD+ cells decreased gradually. The migration of neuron-like cells from the cytospheres after their reseeding was also shown.
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Chung KF, Sicard F, Vukicevic V, et al. Isolation of neural crest derived chromaffin progenitors from adult adrenal medulla. Stem cells, 2009; 27(10): 2602-2613.
Doupe AJ, Landis SC, Patterson PH, Environmental influences in the development of neural crest derivatives: glucocorticoids, growth factors, and chromaffin cell plasticity. The Journal of Neuroscience, 1985; V. 5(8): 2119-2142.
O'Brien JA, Lummis SC. Diolistic labeling of neuronal cultures and intact tissue using a hand-held gene gun. Nat Protoc, 2006; 1(3): 1517-1521.
Wiebe JP. Steroidogenesis in rat Leydig cells: changes in activity of 5-ane and 5-ene 3beta-hydroxysteroid dehydrogenases during sexual maturation. Endocrinology, 1976; 98(2): 505-513.
Sidorenkо OS, Bozhok GA, Bilyavskaya SB. et al. Morphofunctional characteristics of newborn piglets adrenal cells culture. Biotechnology, 2012; 5(5): 72-81.
Ehrhart-Bornstein M, Vukicevic V, Chung KF. et al. Chromaffin Progenitor Cells from the Adrenal Medulla. Cell Mol Neurobiol, 2010; 30(8): 1417-1423.
Santana MM, Chung KF, Vukicevic V. et al. Isolation, Characterization and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla. Stem Cells Transl Med, 2012; 1(11): 783-791.
Unsicker K, Krisch B, Otten U, Thoenen H. Nerve growth factor-induced fiber outgrowth from isolated rat adrenal chromaffin cells: impairment by glucocorticoids. Proc. Natl. Acad. Sci. USA, 1978; 75(7): 3498–3502.
Chu Y, Wu BM, McCabe ER, Dunn JCY. Serum-free cultures of murine adrenal cortical cells. J Pediatr Surg, 2006; 41(12): 2008 – 2012.
Song L, Tang X, Kong Y. et al. The expression of serum steroid sex hormones and steroidogenic enzymes following intraperitoneal administration of dehydroepiandrosterone (DHEA) in male rats. Steroids, 2010; 75(3): 213-218.